pAG306-GPD-ccdB chr I
(Plasmid #41894)

• Purpose: (Empty Backbone)
• Depositing Lab: Dan Gottschling
• Publication: Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi: 10.1038/nature11654. Epub 2012 Nov 21.

Backbone

• Vector backbone: pAG306GPD-ccdB
• Backbone manufacturer: Susan Lindquist (Addgene plasmid# 14140)
• Backbone size (bp): 6972
• Modifications to backbone: Added two regions of homology to S. cerevisiae Chromosome I separated by a Not I restriction site. Plasmid can be integrated into Chr I of S. cerevisiae BY background after digestion with Not I.
• Vector type: Yeast Expression
• Promoter: GPD
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Ampicillin, 25 & 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DB3.1
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pAG306GPD-ccdB was obtained from Addgene
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was generated by ligation of a SmaI-digested fusion PCR product that contained two ~500-base-pair regions of chromosome 1 flanking a NotI site into AatII-digested pAG306-GPD-ccdB.