pSR47S Cm
(Plasmid #41892)

• Purpose: (Empty Backbone)
• Depositing Lab: Howard Steinman
• Publication: Howard Steinman lab unpublished sacB bacterial exchange vectors (unpublished)

Backbone

• Vector backbone: pSR47S
• Backbone size (bp): 6500
• Modifications to backbone: Kanamycin resistance replaced with Chloramphenicol resistance
• Vector type: Bacterial Expression ; Bacterial allelic exchange vector with sacB
• Selectable markers: SacB (sucrose sensitivity)

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5 alpha pir
• Growth instructions: Contains lambda pir-dependent R6K replication origin; requires lambda pir-containing bacteria strain, e.g. DH5a-lambda pir, S17-lambda pir
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• A portion of this plasmid was derived from a plasmid made by: CmR gene derived from pMMB207 vector (bp 1047-1706), received from Howard Shuman, University of Chicago, Chicago, Illinois

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid pSR47S Cm was constructed by removing the KmR region from pSR47S by PCR amplification of the residual plasmid, then cloning in the CmR gene. The CmR gene was amplified from a pMMB207 plasmid by PCR, with restriction sites that allowed cloning into pSR47S that lacked the KmR region.