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pNPTS138 Cm
(Plasmid #41891)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41891 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pNPTS138
  • Backbone size (bp) 5360
  • Modifications to backbone
    Kanamycin resistance replaced with Chloramphenicol resistance
  • Vector type
    Bacterial Expression ; Bacterial allelic exchange vector with sacB
  • Selectable markers
    SacB (sucrose sensitivity)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    CmR gene derived from pMMB207 vector (bp 1047-1706), received from Howard Shuman, University of Chicago, Chicago, Illinois
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid pNPTS138 Cm was constructed by cleavage of pNPTS138 with XhoI, then ligation of the CmR gene, to inactivate the KmR gene.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNPTS138 Cm was a gift from Howard Steinman (Addgene plasmid # 41891 ; http://n2t.net/addgene:41891 ; RRID:Addgene_41891)
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