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Addgene

KaiC CII cat- (E318Q)
(Plasmid #41887)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41887 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    prset-b
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 2200
  • Total vector size (bp) 4468
  • Vector type
    Bacterial Expression ; BL DE3 21

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Growth instructions for expression: Grow 2 L flasks of TB for at 25 C for two days.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kai C CII cat (E318Q)
  • Species
    cyanobacteria
  • Insert Size (bp)
    1500
  • Mutation
    changed amino acid 318 E to Q
  • GenBank ID
    CP000100
  • Promoter t7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BglII (not destroyed)
  • 5′ sequencing primer CGATAAGGATCCGGGACTGGAAGTTCTGTTCCAGGGGCCCATGACTTCCGCTGAGATGACTAGCC
  • 3′ sequencing primer CAAGAAAAAGGGCCGGAGAGCTAAAGATCTGCAGCTGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    KaiC CII cat- (E318Q) was a gift from Michael Rust (Addgene plasmid # 41887 ; http://n2t.net/addgene:41887 ; RRID:Addgene_41887)

  • For your References section:

    Robust and tunable circadian rhythms from differentially sensitive catalytic domains. Phong C, Markson JS, Wilhoite CM, Rust MJ. Proc Natl Acad Sci U S A. 2013 Jan 15;110(3):1124-9. doi: 10.1073/pnas.1212113110. Epub 2012 Dec 31. 10.1073/pnas.1212113110 PubMed 23277568
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