KaiB-FLAG
(Plasmid #41885)

• Depositing Lab: Michael Rust
• Publication: Phong et al Proc Natl Acad Sci U S A. 2013 Jan 15;110(3):1124-9. doi: 10.1073/pnas.1212113110. Epub 2012 Dec 31.

Backbone

• Vector backbone: pET 47b(+)
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5200
• Total vector size (bp): 5500
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Growth instructions for expression: Grow 1 L flask at 37 C until OD600 is about 0.6. Chill flask for 15 minutes on ice. Add ITPG and grow for 3 hours at 30C
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Kai B
• Species: cyanobacteria
• Insert Size (bp): 331
• GenBank ID: CP000100
• Promoter: t7 promoter
• Tag / Fusion Protein:
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: ccaggatcCGATGAGCCCTCGTAAAACCTACATTC
• 3′ sequencing primer: CAAGATTCCGACGACTTCTAATAGAAGCTtgcgg

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Plasmid originally cloned in Rust et al., Science, 318, 809-812 (2007).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry