pCXLE-mp53DD
(Plasmid #41859)

• Purpose: Non-integrating (episomal) expression of mouse p53DD - p53 carboxy-terminal dominant-negative fragment
• Depositing Lab: Shinya Yamanaka
• Publication: Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.

Backbone

• Vector backbone: pCXLE
• Backbone size w/o insert (bp): 10180
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Trp 53
• Alt name: p53
• Alt name: Tp53
• Species: M. musculus (mouse)
• Insert Size (bp): 334
• Mutation: Δ40-903
• GenBank ID: NM_011640
• Entrez Gene: Trp53 (a.k.a. Tp53, bbl, bfy, bhy, p44, p53)
• Promoter: CAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pCAG-F
• 3′ sequencing primer: WPRE-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pCEP4 is from Invitrogen. CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry