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Addgene

pCT302
(Plasmid #41845)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41845 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCT
  • Backbone size w/o insert (bp) 6142
  • Total vector size (bp) 6970
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    4-4-20
  • Species
    Synthetic
  • Insert Size (bp)
    765
  • Tags / Fusion Proteins
    • HA (N terminal on backbone)
    • c-myc (C terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCT302 was a gift from Dane Wittrup (Addgene plasmid # 41845 ; http://n2t.net/addgene:41845 ; RRID:Addgene_41845)

  • For your References section:

    Substantial energetic improvement with minimal structural perturbation in a high affinity mutant antibody. Midelfort KS, Hernandez HH, Lippow SM, Tidor B, Drennan CL, Wittrup KD. J Mol Biol. 2004 Oct 22;343(3):685-701. 10.1016/j.jmb.2004.08.019 PubMed 15465055
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