pCT-4M5.3
(Plasmid
#41844)
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 41844 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCT
- Backbone size w/o insert (bp) 6142
- Total vector size (bp) 6973
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberLow Copy
Gene/Insert
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Gene/Insert name4M5.3
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SpeciesSynthetic
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Insert Size (bp)768
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Tags
/ Fusion Proteins
- HA (N terminal on backbone)
- c-myc (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCT-4M5.3 was a gift from Dane Wittrup (Addgene plasmid # 41844 ; http://n2t.net/addgene:41844 ; RRID:Addgene_41844) -
For your References section:
Substantial energetic improvement with minimal structural perturbation in a high affinity mutant antibody. Midelfort KS, Hernandez HH, Lippow SM, Tidor B, Drennan CL, Wittrup KD. J Mol Biol. 2004 Oct 22;343(3):685-701. 10.1016/j.jmb.2004.08.019 PubMed 15465055
