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Addgene

pSM1959
(Plasmid #41837)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41837 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS425
  • Total vector size (bp) 10476
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SEC63-mRFP
  • Species
    S. cerevisiae (budding yeast)
  • Tag / Fusion Protein
    • mRFP (C terminal on insert)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that Addgene's quality control sequence shows several discrepancies with depositor's reference sequence. These are in vector region and should not affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSM1959 was a gift from Susan Michaelis (Addgene plasmid # 41837 ; http://n2t.net/addgene:41837 ; RRID:Addgene_41837)

  • For your References section:

    Degradation of a cytosolic protein requires endoplasmic reticulum-associated degradation machinery. Metzger MB, Maurer MJ, Dancy BM, Michaelis S. J Biol Chem. 2008 Nov 21;283(47):32302-16. doi: 10.1074/jbc.M806424200. Epub 2008 Sep 23. 10.1074/jbc.M806424200 PubMed 18812321
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