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Addgene

GoldyTALEN-ppp1cab TAL1
(Plasmid #41835)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41835 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    RCIscript-GoldyTALEN
  • Backbone manufacturer
    Dan Carlson (Addgene plasmid# 38142)
  • Backbone size w/o insert (bp) 4651
  • Total vector size (bp) 5841
  • Vector type
    cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    protein phosphatase 1, catalytic subunit, alpha isoform b
  • Alt name
    ppp1cab
  • Species
    D. rerio (zebrafish)
  • GenBank ID
    NM_212710
  • Entrez Gene
    ppp1cab (a.k.a. Ppp1ca, fb18b03, fd20h04, wu:fb18b03, wu:fd20h04, wu:fl22a03, zgc:55744, zgc:76940)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The ppp1cab TALEN recognition sequences are: left TALEN 5′-CCACCAGAGAGTAACT-3′ and right TALEN 5′-GCCTCTGTCAACATAGT-3′. Between the two binding sites is a 15-bp spacer containing a BsII site (ACCTATTTCTGGGAG).

See Addgene plasmid# 41836 and the associated publication for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GoldyTALEN-ppp1cab TAL1 was a gift from Jeffrey Essner (Addgene plasmid # 41835 ; http://n2t.net/addgene:41835 ; RRID:Addgene_41835)

  • For your References section:

    In vivo genome editing using a high-efficiency TALEN system. Bedell VM, Wang Y, Campbell JM, Poshusta TL, Starker CG, Krug RG 2nd, Tan W, Penheiter SG, Ma AC, Leung AY, Fahrenkrug SC, Carlson DF, Voytas DF, Clark KJ, Essner JJ, Ekker SC. Nature. 2012 Nov 1;491(7422):114-8. doi: 10.1038/nature11537. Epub 2012 Sep 23. 10.1038/nature11537 PubMed 23000899
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