pAAH6
(Plasmid #41830)

• Purpose: (Empty Backbone) Used as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for ClonNAT resistance
• Depositing Lab: Melissa Moore
• Publication: Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

Backbone

• Vector backbone: pSET7-26b
• Backbone manufacturer: Covalys
• Backbone size (bp): 3724
• Modifications to backbone: Contains the gene for the SNAP tag along with the clonNAT resistance cassette from pAG25 The nourseothricin/ClonNAT cassette was removed from pAG25 (Euroscarf) by digestion with NotI and SacI and ligated into those same sites of the pSET7-26b SNAP plasmid from Covalys/New England Biolabs.
• Vector type: Bacterial Expression, Yeast Expression ; PCR template
• Promoter: T7,TEF
• Selectable markers: clonNAT/nourseothricin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Unknown

Cloning Information

• 3′ sequencing primer: T7 terminator

Resource Information

• A portion of this plasmid was derived from a plasmid made by: SNAP tag was purchased from NEB.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry