pAAH5
(Plasmid #41829)

• Purpose: (Empty Backbone) Used as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for hygromycin resistance.
• Depositing Lab: Melissa Moore
• Publication: Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

Backbone

• Vector backbone: pSET7-26b
• Backbone manufacturer: Covalys
• Backbone size (bp): 3724
• Modifications to backbone: Contains the gene for the SNAP tag along with the hygromycin resistance cassette from pAG32 Made by digestion of pAG32 (Euroscarf) with BamHI and EcoRV to remove the hygromycin cassette and ligation into the SNAP expression plasmid pSET7-26b (Covalys/New England Biolabs)
• Vector type: Bacterial Expression, Yeast Expression ; PCR template
• Promoter: T7,TEF
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Unknown

Cloning Information

• 3′ sequencing primer: T7 terminator

Resource Information

• A portion of this plasmid was derived from a plasmid made by: SNAP tag obtained from Covalys/NEB

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry