pAAH4
(Plasmid
#41828)
-
Purpose(Empty Backbone) Used as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for phleomycin resistance
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 41828 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepUG66
- Backbone size (bp) 3580
-
Modifications to backboneContains the gene for the E. coli dihydrofolate reductase (DHFR) Made by Quikchange mutagenesis of pAAH3 to insert a stop codon before the histag
-
Vector typeYeast Expression ; PCR template
- Promoter TEF
-
Selectable markersphleomycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)Top10
-
Copy numberUnknown
Cloning Information
- 5′ sequencing primer T7
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byVirginia Cornish
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pAAH4 was a gift from Melissa Moore (Addgene plasmid # 41828 ; http://n2t.net/addgene:41828 ; RRID:Addgene_41828) -
For your References section:
Ordered and dynamic assembly of single spliceosomes. Hoskins AA, Friedman LJ, Gallagher SS, Crawford DJ, Anderson EG, Wombacher R, Ramirez N, Cornish VW, Gelles J, Moore MJ. Science. 2011 Mar 11;331(6022):1289-95. 10.1126/science.1198830 PubMed 21393538
