pAAH3
(Plasmid #41827)

• Purpose: (Empty Backbone) Used as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for phleomycin resistance.
• Depositing Lab: Melissa Moore
• Publication: Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

Backbone

• Vector backbone: pUG66
• Backbone size (bp): 3580
• Modifications to backbone: Contains the gene for the E. coli dihydrofolate reductase (DHFR) with a c-terminal histag Made by removal of the DHFR gene from pAED4-DHFR (L. W. Miller, Y. Cai, M. P. Sheetz, V. W. Cornish, Nat. Methods 2, 255 (2005)) by digestion with NdeI and HindIII and ligation into the pUG66 plasmid (Euroscarf)
• Vector type: Yeast Expression ; PCR template
• Promoter: TEF
• Selectable markers: phleomycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• 3′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Virginia Cornish

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry