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Addgene

D10 pAcGP67a
(Plasmid #41812)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41812 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAcGP67a
  • Backbone manufacturer
    BD
  • Backbone size w/o insert (bp) 9761
  • Total vector size (bp) 10000
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    D10 Super-2
  • Alt name
    Super-2
  • Alt name
    D10
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    429
  • Entrez Gene
    IL2 (a.k.a. IL-2, TCGF, lymphokine)
  • Promoter Polyhedron
  • Tag / Fusion Protein
    • 8x His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer PolyhedronFor
  • 3′ sequencing primer PolyhedronRev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

D10 has the following mutations compared to wildtype IL-2: Q74H, L80F, R81F, L85V, I86V, I92F

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    D10 pAcGP67a was a gift from Chris Garcia (Addgene plasmid # 41812 ; http://n2t.net/addgene:41812 ; RRID:Addgene_41812)

  • For your References section:

    Exploiting a natural conformational switch to engineer an interleukin-2 'superkine'. Levin AM, Bates DL, Ring AM, Krieg C, Lin JT, Su L, Moraga I, Raeber ME, Bowman GR, Novick P, Pande VS, Fathman CG, Boyman O, Garcia KC. Nature. 2012 Mar 25;484(7395):529-33. doi: 10.1038/nature10975. 10.1038/nature10975 PubMed 22446627
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