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Addgene

pDM8
(Plasmid #41758)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41758 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pT7-7
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    kinesin heavy chain
  • Species
    D. melanogaster (fly); E. coli
  • Mutation
    residues 1-401; C45S C338S S364C C451S
  • Entrez Gene
    Khc (a.k.a. Dmel_CG7765, 2R6, CG7765, DK, DKH, Dm KHC, DmK, DmKHC, Dmel\CG7765, Dmkin, KHC, KIF 5A, KIF5, KIF5B, KIN, Kif5, Kin, Kin-1, Kinesin, Kinesin-1, khc, kin, kinesin, kinesin-1, l(2)W12, l(2)k13219, l(2)k13314, l(2R)W12, pgs)
  • Tags / Fusion Proteins
    • bccp (C terminal on insert)
    • His6 (C terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Larry Goldstein; John Cronan

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDM8 was a gift from Jeff Gelles (Addgene plasmid # 41758 ; http://n2t.net/addgene:41758 ; RRID:Addgene_41758)

  • For your References section:

    FRET measurements of kinesin neck orientation reveal a structural basis for processivity and asymmetry. Martin DS, Fathi R, Mitchison TJ, Gelles J. Proc Natl Acad Sci U S A. 2010 Mar 23;107(12):5453-8. Epub 2010 Mar 8. 10.1073/pnas.0914924107 PubMed 20212149
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