Skip to main content
Addgene

pTTR210K
(Plasmid #41750)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41750 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pT7-7
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    kinesin heavy chain
  • Species
    D. melanogaster (fly); E. coli
  • Mutation
    residues 1-401; R210K
  • Entrez Gene
    Khc (a.k.a. Dmel_CG7765, 2R6, CG7765, DK, DKH, Dm KHC, DmK, DmKHC, Dmel\CG7765, Dmkin, KHC, KIF 5A, KIF5, KIF5B, KIN, Kif5, Kin, Kin-1, Kinesin, Kinesin-1, khc, kin, kinesin, kinesin-1, l(2)W12, l(2)k13219, l(2)k13314, l(2R)W12, pgs)
  • Tags / Fusion Proteins
    • bccp (C terminal on insert)
    • His6 (C terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Larry Goldstein

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTTR210K was a gift from Jeff Gelles (Addgene plasmid # 41750 ; http://n2t.net/addgene:41750 ; RRID:Addgene_41750)

  • For your References section:

    Processive movement by a kinesin heterodimer with an inactivating mutation in one head. Thoresen T, Gelles J. Biochemistry. 2008 Sep 9;47(36):9514-21. Epub 2008 Aug 15. 10.1021/bi800747e PubMed 18702529
Related items: