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Addgene

pSigma54-4
(Plasmid #41748)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41748 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b
  • Backbone manufacturer
    novagen
  • Backbone size w/o insert (bp) 5708
  • Total vector size (bp) 7139
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    rpoN
  • Species
    E. coli
  • Mutation
    c198a c307g c346a
  • Entrez Gene
    rpoN (a.k.a. b3202, ECK3191, JW3169, glnF, ntrA)
  • Tag / Fusion Protein
    • n-terminal HHHHHHssClvprgsh

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Karsten Rippe

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSigma54-4 was a gift from Jeff Gelles (Addgene plasmid # 41748 ; http://n2t.net/addgene:41748 ; RRID:Addgene_41748)

  • For your References section:

    Mechanism of transcription initiation at an activator-dependent promoter defined by single-molecule observation. Friedman LJ, Gelles J. Cell. 2012 Feb 17;148(4):679-89. doi: 10.1016/j.cell.2012.01.018. 10.1016/j.cell.2012.01.018 PubMed 22341441
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