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Addgene

HA-EGFP-HaloTag2
(Plasmid #41742)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41742 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PCDNA5
  • Backbone manufacturer
    INVITROGEN
  • Backbone size w/o insert (bp) 5000
  • Modifications to backbone
    Kozak Consensus Sequence added
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Halotag 2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    885
  • Promoter CMV

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
  • 3′ sequencing primer TAGAAGGCACAGTCGAGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Alternate plasmid name: pCDNA5-KGH2

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-EGFP-HaloTag2 was a gift from Craig Crews (Addgene plasmid # 41742 ; http://n2t.net/addgene:41742 ; RRID:Addgene_41742)

  • For your References section:

    Small-molecule hydrophobic tagging-induced degradation of HaloTag fusion proteins. Neklesa TK, Tae HS, Schneekloth AR, Stulberg MJ, Corson TW, Sundberg TB, Raina K, Holley SA, Crews CM. Nat Chem Biol. 2011 Jul 3;7(8):538-43. doi: 10.1038/nchembio.597. 10.1038/nchembio.597 PubMed 21725302
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