pCS2 Notch1 LNG (CC→SS)-6MT
(Plasmid #41739)

• Depositing Lab: Raphael Kopan
• Publication: Mumm et al Mol Cell. 2000 Feb;5(2):197-206.

Backbone

• Vector backbone: pCS2+MT
• Backbone size w/o insert (bp): 4352
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Notch-1 Lin-Notch-glp (LNG) repeat-containing construct, CC->SS
• Alt name: Notch1
• Alt name: N1 Lin-Notch-glp (LNG) repeat-containing construct, CC->SS
• Alt name: N1
• Species: M. musculus (mouse)
• Mutation: Contains murine Notch-1 leader peptide (aa 1-23) and then protein from the extracellular lin-Notch-glp (LNG) repeats to residue 2184 (aa 1448-2184); C1675S, C1682S
• GenBank ID: AAM28905.1 AF508809_1
• Entrez Gene: Notch1 (a.k.a. 9930111A19Rik, Mis6, N1, Tan1, lin-12)
• Tag / Fusion Protein:
  • 6xMyc (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: Sp6
• 3′ sequencing primer: EBV-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Full length Notch-1 cDNA from Jeffrey S. Nye (Northwestern University School of Medicine, Chicago, IL)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Alternate plasmid name: pCS2 NLNR CC>SS-6MT

All Notch1 constructs deposited here were cloned into the pCS2+MT vector (Rupp et al., 1994; Turner and Weintraub, 1994) and have the C-terminal 348 residues (aa 2185 to C terminus) of Notch replaced with a hexameric myc tag to facilitate biochemical analysis. PCR mutagenesis was used to create the C1675S and C1682S mutations in LNR (Addgene plasmid #41738).