pCS2 Notch1 LNG-6MT
(Plasmid
#41738)
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Depositing Labs
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 41738 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCS2+MT
- Backbone size w/o insert (bp) 4352
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNotch-1 Lin-Notch-glp (LNG) repeat-containing construct
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Alt nameNotch1
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Alt nameN1 Lin-Notch-glp (LNG) repeat-containing construct
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Alt nameN1
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SpeciesM. musculus (mouse)
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MutationContains murine Notch-1 leader peptide (aa 1-23) and then protein from the extracellular lin-Notch-glp (LNG) repeats to residue 2184 (aa 1448-2184)
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GenBank IDAAM28905.1 AF508809_1
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Entrez GeneNotch1 (a.k.a. 9930111A19Rik, Mis6, N1, Tan1, lin-12)
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Tag
/ Fusion Protein
- 6xMyc (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Sp6
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byFull length Notch-1 cDNA from Jeffrey S. Nye (Northwestern University School of Medicine, Chicago, IL)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Alternate plasmid name: pCS2 NLNR-6MT
All Notch1 constructs deposited here were cloned into the pCS2+MT vector (Rupp et al., 1994; Turner and Weintraub, 1994) and have the C-terminal 348 residues (aa 2185 to C terminus) of Notch replaced with a hexameric myc tag to facilitate biochemical analysis.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2 Notch1 LNG-6MT was a gift from Raphael Kopan & Jeffrey Nye (Addgene plasmid # 41738 ; http://n2t.net/addgene:41738 ; RRID:Addgene_41738) -
For your References section:
Signal transduction by activated mNotch: importance of proteolytic processing and its regulation by the extracellular domain. Kopan R, Schroeter EH, Weintraub H, Nye JS. Proc Natl Acad Sci U S A. 1996 Feb 20;93(4):1683-8. 10.1073/pnas.93.4.1683 PubMed 8643690
Map uploaded by the depositor.