pCS2 Notch1 ΔEMV-6MT
(Plasmid #41737)

• Depositing Labs: Raphael Kopan, Jeffrey Nye
• Publication: Kopan et al Proc Natl Acad Sci U S A. 1996 Feb 20;93(4):1683-8.

Backbone

• Vector backbone: pCS2+MT
• Backbone size w/o insert (bp): 4352
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Notch-1 Extracellular-domain-deleted construct
• Alt name: Notch1
• Alt name: N1 Extracellular-domain-deleted construct
• Alt name: N1
• Species: M. musculus (mouse)
• Mutation: Contains murine Notch-1 protein fragment lacking the extracellular region [contains murine Notch-1 leader peptide (aa 1-23) and then aa 1704-2184]; M1727V
• GenBank ID: AAM28905.1 AF508809_1
• Entrez Gene: Notch1 (a.k.a. 9930111A19Rik, Mis6, N1, Tan1, lin-12)
• Tag / Fusion Protein:
  • 6xMyc (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: Sp6
• 3′ sequencing primer: EBV-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Full length Notch-1 cDNA from Jeffrey S. Nye (Northwestern University School of Medicine, Chicago, IL)
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Alternate plasmid name: pCS2 NΔE-6MT

All Notch1 constructs deposited here were cloned into the pCS2+MT vector (Rupp et al., 1994; Turner and Weintraub, 1994) and have the C-terminal 348 residues (aa 2185 to C terminus) of Notch replaced with a hexameric myc tag to facilitate biochemical analysis.