pLew100_myc_BirA*
(Plasmid #41716)

• Purpose: (Empty Backbone)
• Depositing Lab: Graham Warren
• Publication: Morriswood et al Eukaryot Cell. 2012 Dec 21.

Backbone

• Vector backbone: pLew100
• Backbone size (bp): 7417
• Modifications to backbone: Contains myc-BirA* module (R118G)
• Vector type: T. brucei expression
• Promoter: PARP
• Selectable markers: Phleomycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The pLew100_myc-BirA* plasmid is derived from the pcDNA3.1(-)_myc-BirA* (http://www.addgene.org/36047/) plasmid originating in the Roux lab (Sanford Health, SD, USA). It contains the myc-BirA* module cloned in the T. brucei pLew100 expression vector, with some extra restriction sites to facilitate cloning.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

If you use this plasmid in a publication, please also cite the Morriswood paper as well as the following article:
A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells. Roux et al (J Cell Biol. 2012 Mar 12). PubMed - https://www.ncbi.nlm.nih.gov/pubmed/22412018 .