Skip to main content
Addgene

pEXP5-NT-AlleyCat
(Plasmid #41715)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41715 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pEXP5-NT/TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 2745
  • Total vector size (bp) 2966
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mutated C-terminal portion of calmodulin gene (M76-K148)
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    221
  • Mutation
    F92E
  • Promoter T7
  • Tag / Fusion Protein
    • Polyhistidine (6×His) region, TEV recognition site (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEXP5-NT-AlleyCat was a gift from William DeGrado & Ivan Korendovych (Addgene plasmid # 41715 ; http://n2t.net/addgene:41715 ; RRID:Addgene_41715)

  • For your References section:

    Design of a switchable eliminase. Korendovych IV, Kulp DW, Wu Y, Cheng H, Roder H, DeGrado WF. Proc Natl Acad Sci U S A. 2011 Apr 26;108(17):6823-7. Epub 2011 Apr 11. 10.1073/pnas.1018191108 PubMed 21482808
Related items: