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Addgene

FH-Tet2-pEF
(Plasmid #41710)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41710 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEF1a
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6174
  • Total vector size (bp) 11969
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tet methylcytosine dioxygenase 2
  • Alt name
    Tet2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    5835
  • GenBank ID
    XM_006501286.1
  • Entrez Gene
    Tet2 (a.k.a. Ayu17-449, E130014J05Rik, mKIAA1546)
  • Promoter EF1a
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer EF1a-F 5'-TCAAGCCTCAGACAGTGGTTC-3'
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FH-Tet2-pEF was a gift from Anjana Rao (Addgene plasmid # 41710 ; http://n2t.net/addgene:41710 ; RRID:Addgene_41710)

  • For your References section:

    Impaired hydroxylation of 5-methylcytosine in myeloid cancers with mutant TET2. Ko M, Huang Y, Jankowska AM, Pape UJ, Tahiliani M, Bandukwala HS, An J, Lamperti ED, Koh KP, Ganetzky R, Liu XS, Aravind L, Agarwal S, Maciejewski JP, Rao A. Nature. 2010 Dec 9;468(7325):839-43. doi: 10.1038/nature09586. 10.1038/nature09586 PubMed 21057493
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