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Addgene

EcNR2.dnaG.Q576A
(Bacterial strain #41700)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Bacterial Strain 41700 Bacteria in agar stab 1 $85
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Backbone

  • Vector backbone
    genome

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Nuc5-.dnaG.Q576A
  • Growth instructions
    Do not grow above 34 C because lambda red will begin to have leaky expression.
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    E. coli MG1655 ΔmutS::cat Δ(ybhB-bioAB)::[λcI857 N(cro-ea59)::tetR-bla] dnaG.Q576A

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EcNR2.dnaG.Q576A was a gift from George Church (Addgene plasmid # 41700)

  • For your References section:

    Manipulating replisome dynamics to enhance lambda Red-mediated multiplex genome engineering. Lajoie MJ, Gregg CJ, Mosberg JA, Washington GC, Church GM. Nucleic Acids Res. 2012 Aug 16. 10.1093/nar/gks751 PubMed 22904085
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