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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 41684 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRSFDuet-1
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Backbone manufacturernovagen
- Total vector size (bp) 4392
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Modifications to backboneremoved the second MCS of pRSFDuet-1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNpuDnaE intein
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Alt namecis-splicing DnaE intein with GB1 as exteins
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SpeciesNostoc punctiforme
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Insert Size (bp)825
- Promoter T7
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Tags
/ Fusion Proteins
- GB1 (N terminal on insert)
- GB1 (C terminal on insert)
- His-tag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSKDuet16 was a gift from Hideo Iwai (Addgene plasmid # 41684 ; http://n2t.net/addgene:41684 ; RRID:Addgene_41684) -
For your References section:
Evaluation and comparison of protein splicing by exogenous inteins with foreign exteins in Escherichia coli. Ellila S, Jurvansuu JM, Iwai H. FEBS Lett. 2011 Oct 10. 10.1016/j.febslet.2011.10.005 PubMed 22001202
Map uploaded by the depositor.
