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CFP_Rad_pcDNA4_HisMaxC
(Plasmid #41654)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41654 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4/His-Max C
  • Backbone manufacturer
    Invitrogen
  • Total vector size (bp) 6883
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Rad, GTP binding proteins (RGK)
  • Alt name
    Rad
  • Alt name
    Rrad
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    921
  • GenBank ID
    NM_019662
  • Entrez Gene
    Rrad (a.k.a. REM3, Rad)
  • Promoter CMV
  • Tag / Fusion Protein
    • CFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7_promoter
  • 3′ sequencing primer BGH Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CFP_Rad_pcDNA4_HisMaxC was a gift from Henry Colecraft (Addgene plasmid # 41654 ; http://n2t.net/addgene:41654 ; RRID:Addgene_41654)

  • For your References section:

    Distinct RGK GTPases differentially use alpha1- and auxiliary beta-binding-dependent mechanisms to inhibit CaV1.2/CaV2.2 channels. Yang T, Puckerin A, Colecraft HM. PLoS One. 2012;7(5):e37079. Epub 2012 May 10. 10.1371/journal.pone.0037079 PubMed 22590648
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