CFP_Gem_pcDNA4_HisMaxC
(Plasmid #41653)

• Depositing Lab: Henry Colecraft
• Publication: Yang et al PLoS One. 2012;7(5):e37079. Epub 2012 May 10.

Backbone

• Vector backbone: pcDNA4/His-Max C
• Backbone manufacturer: Invitrogen
• Total vector size (bp): 6851
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Gem, GTP binding proteins (RGK)
• Alt name: Gem
• Species: H. sapiens (human)
• Insert Size (bp): 886
• GenBank ID: NM_181702
• Entrez Gene: GEM (a.k.a. KIR)
• Promoter: CMV
• Tag / Fusion Protein:
  • CFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7_promoter
• 3′ sequencing primer: BGH Reverse

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry