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TMEM216-mCherry
(Plasmid #41633)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41633 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Modifications to backbone
    added C-terminal mCherry
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Transmembrane Protein 216
  • Alt name
    TMEM216
  • Species
    H. sapiens (human)
  • Entrez Gene
    TMEM216 (a.k.a. HSPC244)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer mCherry-R for insert; BGH-rev for vector
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See Table S6 of Supporting Online Material from associated publication for TMEM216 reference sequence.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TMEM216-mCherry was a gift from Joseph Gleeson (Addgene plasmid # 41633 ; http://n2t.net/addgene:41633 ; RRID:Addgene_41633)

  • For your References section:

    Evolutionarily assembled cis-regulatory module at a human ciliopathy locus. Lee JH, Silhavy JL, Lee JE, Al-Gazali L, Thomas S, Davis EE, Bielas SL, Hill KJ, Iannicelli M, Brancati F, Gabriel SB, Russ C, Logan CV, Sharif SM, Bennett CP, Abe M, Hildebrandt F, Diplas BH, Attie-Bitach T, Katsanis N, Rajab A, Koul R, Sztriha L, Waters ER, Ferro-Novick S, Woods CG, Johnson CA, Valente EM, Zaki MS, Gleeson JG. Science. 2012 Feb 24;335(6071):966-9. Epub 2012 Jan 26. 10.1126/science.1213506 PubMed 22282472
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