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Addgene

pSH-hPXR-LBD-K277Q
(Plasmid #41620)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41620 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pSH
  • Backbone manufacturer
    ATCC (Item #77476)
  • Backbone size w/o insert (bp) 5900
  • Total vector size (bp) 6900
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hPXR-LBD-K277Q
  • Alt name
    PXR
  • Alt name
    NR1I2
  • Alt name
    SXR
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1000
  • Mutation
    contains human PXR ligand binding domain (LBD, aa 107-434), K277Q
  • GenBank ID
    AF061056.1 AAD05436.1
  • Entrez Gene
    NR1I2 (a.k.a. BXR, ONR1, PAR, PAR1, PAR2, PARq, PRR, PXR, SAR, SXR)
  • Promoter pEN1 (chimeric HIV-1 LTR enhancer and HTLV-I LTR promoter)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Wild-type hPXR cDNA acquired from Ronald Evans (Salk Institute, La Jolla, CA)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Human PXR ligand binding domain (LBD, aa 107-434) was cloned into vector pSH. Mutations were made and confirmed by sequencing.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSH-hPXR-LBD-K277Q was a gift from Sridhar Mani (Addgene plasmid # 41620 ; http://n2t.net/addgene:41620 ; RRID:Addgene_41620)