pSH-hPXR-LBD-K277Q
(Plasmid
#41620)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 41620 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSH
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Backbone manufacturerATCC (Item #77476)
- Backbone size w/o insert (bp) 5900
- Total vector size (bp) 6900
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehPXR-LBD-K277Q
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Alt namePXR
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Alt nameNR1I2
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Alt nameSXR
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SpeciesH. sapiens (human)
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Insert Size (bp)1000
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Mutationcontains human PXR ligand binding domain (LBD, aa 107-434), K277Q
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GenBank IDAF061056.1 AAD05436.1
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Entrez GeneNR1I2 (a.k.a. BXR, ONR1, PAR, PAR1, PAR2, PARq, PRR, PXR, SAR, SXR)
- Promoter pEN1 (chimeric HIV-1 LTR enhancer and HTLV-I LTR promoter)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Unknown
- 3′ sequencing primer SV40-pA-R (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byWild-type hPXR cDNA acquired from Ronald Evans (Salk Institute, La Jolla, CA)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Human PXR ligand binding domain (LBD, aa 107-434) was cloned into vector pSH. Mutations were made and confirmed by sequencing.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSH-hPXR-LBD-K277Q was a gift from Sridhar Mani (Addgene plasmid # 41620 ; http://n2t.net/addgene:41620 ; RRID:Addgene_41620)