pCDNA3-hPXR-FL-K277Q
(Plasmid #41618)

• Depositing Lab: Sridhar Mani
• Publication: Mani lab mutant human PXR expression plasmids (unpublished)

Backbone

• Vector backbone: pCDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 6700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hPXR-FL-K277Q
• Alt name: PXR
• Alt name: NR1I2
• Alt name: SXR
• Species: H. sapiens (human)
• Insert Size (bp): 1300
• Mutation: N10D and K277Q
• GenBank ID: AF061056.1 AAD05436.1
• Entrez Gene: NR1I2 (a.k.a. BXR, ONR1, PAR, PAR1, PAR2, PARq, PRR, PXR, SAR, SXR)
• Promoter: CMV

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Wild-type hPXR cDNA acquired from Ronald Evans (Salk Institute, La Jolla, CA)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Full length (aa 1-434) human PXR was cloned into pCDNA3. Mutations were made and confirmed by sequencing.

PXR sequence contains N10D mutation when compared to GenBank entry AAD05436.1 (NP_003880.3)