pFA6a-kanMX6-PGAL1-3HA
(Plasmid #41608)

• Depositing Lab: John Pringle
• Publication: Longtine et al Yeast. 1998 Jul;14(10):953-61.

Backbone

• Vector backbone: pFA6a
• Vector type: Yeast genomic targeting
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GAL1 promoter
• Alt name: GAL1
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 2081
• Entrez Gene: GAL1 (a.k.a. YBR020W)
• Tags / Fusion Proteins:
  • A. gossypii translation elongation factor 1a gene terminator (N terminal on insert)
  • KanR reverse orientation (N terminal on insert)
  • A. gossypii translation elongation factor 1a gene promoter (N terminal on insert)
  • 3xHA (C terminal on insert)
  • yeast ADH1 terminator (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: PacI (not destroyed)
• 5′ sequencing primer: T7_promoter
• 3′ sequencing primer: Sp6_primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry