pFA6a-GFP(S65T)-His3MX6
(Plasmid #41598)

• Depositing Lab: John Pringle
• Publication: Longtine et al Yeast. 1998 Jul;14(10):953-61.

Backbone

• Vector backbone: pFA6a-kanMX6
• Backbone size w/o insert (bp): 3938
• Vector type: Yeast Expression ; Yeast genomic targeting
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: His3
• Alt name: k-HIS3
• Alt name: SKHIS3
• Species: S. kluyveri
• Insert Size (bp): 2348
• Promoter: TEF
• Tags / Fusion Proteins:
  • GFP (S65T) (N terminal on insert)
  • yeast ADH1 terminator (N terminal on insert)
  • A. gossypii translation elongation factor 1a gene promoter (N terminal on insert)
  • A. gossypii translation elongation factor 1a gene terminator (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: PacI (not destroyed)
• 3′ cloning site: PmeI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry