pFA6a-His3MX6
(Plasmid #41596)

• Depositing Lab: John Pringle
• Publication: Longtine et al Yeast. 1998 Jul;14(10):953-61.

Backbone

• Vector backbone: pFA6a
• Backbone size w/o insert (bp): 2300
• Vector type: Yeast genomic targeting
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: His3
• Alt name: k-HIS3
• Alt name: SKHIS3
• Species: S. kluyveri
• Insert Size (bp): 1403
• Tags / Fusion Proteins:
  • A. gossypii translation elongation factor 1a gene promoter (N terminal on insert)
  • A. gossypii translation elongation factor 1a gene terminator (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: PmeI (not destroyed)
• 5′ sequencing primer: Sp6_primer
• 3′ sequencing primer: T7_promoter

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the associated publication contains important primer information for the intended application of this plasmid, but refers to a version of the plasmid containing the S. pombe HIS5 gene while this plasmid contains the S. kluyveri HIS3 gene. The generation of both plasmids is described in Wach, A., Brachat, A., Alberti-Segui, C., Rebischung, C. and Philippsen, P. (1997). Heterologous HIS3 marker and GFP reporter modules for PCR-targeting in Saccharomyces cerevisiae. Yeast 13, 1065–1075. Pubmed ID: 9290211.