pCAGGS-Flag-hsDicer (E1564A)
(Plasmid
#41587)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 41587 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCAGGS
- Backbone size w/o insert (bp) 4790
- Total vector size (bp) 10528
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Vector typeMammalian Expression
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Selectable markersNone
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10/P3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehsDicer
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Alt nameDicer1
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SpeciesH. sapiens (human)
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Insert Size (bp)5796
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MutationE1564A
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GenBank IDNM_177438 NM_030621
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Entrez GeneDICER1 (a.k.a. DCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2, aviD)
- Promoter CMV
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer Custom
- 3′ sequencing primer Custom (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAGGS-Flag-hsDicer (E1564A) was a gift from Phil Sharp (Addgene plasmid # 41587 ; http://n2t.net/addgene:41587 ; RRID:Addgene_41587) -
For your References section:
In vivo structure-function analysis of human Dicer reveals directional processing of precursor miRNAs. Gurtan AM, Lu V, Bhutkar A, Sharp PA. RNA. 2012 Jun;18(6):1116-22. Epub 2012 Apr 30. 10.1261/rna.032680.112 PubMed 22546613
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/57/46/c57ba964-43a2-11e2-a59f-003048dd6500.png.940x940_q85_autocrop.png)
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/45/97/c5bc351a-43a2-11e2-a59f-003048dd6500.png.940x940_q85_autocrop.png)