pCAGGS-Flag-hsDicer (D1320A/D1709A)
(Plasmid #41585)

• Depositing Lab: Phil Sharp
• Publication: Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 Apr 30.

Backbone

• Vector backbone: pCAGGS
• Backbone size w/o insert (bp): 4790
• Total vector size (bp): 10528
• Vector type: Mammalian Expression
• Selectable markers: None

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10/P3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hsDicer
• Alt name: Dicer1
• Species: H. sapiens (human)
• Insert Size (bp): 5796
• Mutation: D1320A/D1709A
• GenBank ID: NM_177438 NM_030621
• Entrez Gene: DICER1 (a.k.a. DCR1, Dicer, Dicer1e, GLOW, HERNA, K12H4.8-LIKE, MNG1, RMSE2, aviD)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: Custom
• 3′ sequencing primer: Custom

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry