pCAGGS-mCherry
(Plasmid #41583)

• Purpose: (Empty Backbone)
• Depositing Lab: Phil Sharp
• Publication: Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 Apr 30.

Backbone

• Vector backbone: pCAGGS
• Backbone size (bp): 4790
• Modifications to backbone: mCherry inserted into cloning site.
• Vector type: Mammalian Expression
• Promoter: CMV
• Selectable markers: None

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10/P3
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: Custom
• 3′ sequencing primer: Custom

Resource Information

• Articles Citing this Plasmid:
  • 20 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Information for Cloning Grade DNA (Catalog # 41583-DNA.cg) ( Back to top)

Purpose

Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

Delivery

• Amount: 2 µg
• Guaranteed Concentration: 100 ng/µl +/- 5 ng/µl
• Pricing: $105 USD
• Storage: DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here