NTS-PC1
(Plasmid
#41574)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 41574 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePKD1
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Alt namePolycystin-1
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SpeciesH. sapiens (human)
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Entrez GenePKD1 (a.k.a. PBP, PC1, Pc-1, TRPP1)
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Tag
/ Fusion Protein
- Myc/Myc/His (C terminal on backbone)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
5' cloning site: EcoR1
3' cloning site: XhoI
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NTS-PC1 was a gift from Thomas Weimbs (Addgene plasmid # 41574 ; http://n2t.net/addgene:41574 ; RRID:Addgene_41574) -
For your References section:
Polycystin-1 regulates STAT activity by a dual mechanism. Talbot JJ, Shillingford JM, Vasanth S, Doerr N, Mukherjee S, Kinter MT, Watnick T, Weimbs T. Proc Natl Acad Sci U S A. 2011 May 10;108(19):7985-90. Epub 2011 Apr 25. 10.1073/pnas.1103816108 PubMed 21518865