NTM (FLM-PC1Δ137)
(Plasmid #41572)

• Depositing Lab: Thomas Weimbs
• Publication: Talbot et al Proc Natl Acad Sci U S A. 2011 May 10;108(19):7985-90. Epub 2011 Apr 25.

Backbone

• Vector backbone: pcDNA4/TO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5100
• Total vector size (bp): 6268
• Modifications to backbone: stop codon before the NotI restriction site
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PKD1
• Alt name: Polycystin-1
• Species: H. sapiens (human)
• Mutation: sequence of PKD1 FLM from 4078 - 4273
• Entrez Gene: PKD1 (a.k.a. PBP, PC1, Pc-1, TRPP1)
• Tags / Fusion Proteins:
  • CD16.7 (N terminal on insert)
  • CD16.7 (N terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Insert originally obtained from Dr. Gerd Walz, Freiburg, Germany

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

5' cloning site: HindIII
3' cloning site: NotI

Note: stop codon is prior to the myc-His tag. Therefore, no C-terminal tag is expressed