FLM-PC1
(Plasmid
#41567)
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 41567 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA4/TO
-
Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5100
- Total vector size (bp) 6670
-
Vector typeMammalian Expression
-
Selectable markersZeocin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert namePKD1
-
Alt namePolycystin-1
-
SpeciesH. sapiens (human)
-
Mutationsequence from 4078 - 4303 of PKD1
-
Entrez GenePKD1 (a.k.a. PBP, PC1, Pc-1, TRPP1)
-
Tag
/ Fusion Protein
- CD16.7 (N terminal on insert)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byInsert originally obtained from Dr. Gerd Walz, Freiburg, Germany
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
5' cloning site: HindIII
3' cloning site: NotI
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
FLM-PC1 was a gift from Thomas Weimbs (Addgene plasmid # 41567 ; http://n2t.net/addgene:41567 ; RRID:Addgene_41567) -
For your References section:
Polycystin-1 regulates STAT activity by a dual mechanism. Talbot JJ, Shillingford JM, Vasanth S, Doerr N, Mukherjee S, Kinter MT, Watnick T, Weimbs T. Proc Natl Acad Sci U S A. 2011 May 10;108(19):7985-90. Epub 2011 Apr 25. 10.1073/pnas.1103816108 PubMed 21518865