SIRT6
(Plasmid #41565)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: SGC SIRT6 plasmids (unpublished)

Backbone

• Vector backbone: pET28a-LIC
• Backbone manufacturer: SGC
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: SIRT6
• Alt name: sirtuin 6, silent mating type information regulation 2 homolog) 6
• Alt name: PDB ID: 3PKI
• Species: H. sapiens (human)
• Insert Size (bp): 1059
• Mutation: aa 3-353
• GenBank ID: BC005026
• Entrez Gene: SIRT6 (a.k.a. SIR2L6, hSIRT6)
• Promoter: T7
• Tag / Fusion Protein:
  • His Tev (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

http://www.thesgc.org/structures/3PKI/