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Addgene

pNC1136
(Plasmid #41562)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41562 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC118
  • Total vector size (bp) 3119
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ura3-FUS1-UBIYdkGFP*-SpHis5-Tim9
  • Species
    S. cerevisiae (budding yeast), S. pombe (fission yeast); Aequorea victoria
  • Insert Size (bp)
    3833
  • Mutation
    GFP mutations: Phe 64 to Leu, S65 to Thr, V163 to A His 77 codon CAT to CAC; destroys NdeI site

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNC1136 was a gift from Beverly Errede (Addgene plasmid # 41562 ; http://n2t.net/addgene:41562 ; RRID:Addgene_41562)

  • For your References section:

    An improved short-lived fluorescent protein transcriptional reporter for Saccharomyces cerevisiae. Houser JR, Ford E, Chatterjea SM, Maleri S, Elston TC, Errede B. Yeast. 2012 Dec;29(12):519-30. doi: 10.1002/yea.2932. Epub 2012 Nov 21. 10.1002/yea.2932 PubMed 23172645
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