PREX2 pcdna3.1 V5/his
(Plasmid #41555)

• Depositing Lab: Ramon Parsons
• Publication: Fine et al Science. 2009 Sep 4;325(5945):1261-5.

Backbone

• Vector backbone: pcDNA3.1/V5-His
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5523
• Total vector size (bp): 10700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PREX2a
• Alt name: DEPDC2
• Species: H. sapiens (human)
• Insert Size (bp): 5150
• GenBank ID: NM_024870.2
• Entrez Gene: PREX2 (a.k.a. 6230420N16Rik, DEP.2, DEPDC2, P-REX2, PPP1R129)
• Promoter: T7
• Tags / Fusion Proteins:
  • V5 (C terminal on backbone)
  • his (C terminal on backbone)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGHReverse

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry