pETcon(-)
(Plasmid #41522)

• Purpose: (Empty Backbone)
• Depositing Lab: Andrew Scharenberg
• Publication: pETCON Yeast Surface Display Plasmids (unpublished)

Backbone

• Vector backbone: pETcon
• Backbone size (bp): 6139
• Modifications to backbone: Stuffer inserted between NdeI and XhoI (puts Myc tag out of frame to prevent parental contamination). Originally derived from the pCTcon2 vector.
• Vector type: Yeast Expression
• Promoter: GAL1
• Selectable markers: TRP1
• Tags / Fusion Proteins:
  • HA (N terminal on backbone)
  • 3x(G4S) Linker (N terminal on backbone)
  • Stuffer (N terminal on insert)
  • G3S Linker (C terminal on backbone)
  • Myc Tag (C terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: GAL1
• 3′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Derived from pCTcon2 (recieved from Dr. Karl D Wittrup at MIT without an MTA)
• Articles Citing this Plasmid:
  • 14 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry