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Addgene

G6PD/pRK5
(Plasmid #41521)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41521 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    glucose-6-phosphate dehydrogenase
  • Alt name
    G6PD2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1548
  • GenBank ID
    NM_001042351
  • Entrez Gene
    G6PD (a.k.a. G6PD1)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    G6PD/pRK5 was a gift from Xiaolu Yang (Addgene plasmid # 41521 ; http://n2t.net/addgene:41521 ; RRID:Addgene_41521)

  • For your References section:

    p53 regulates biosynthesis through direct inactivation of glucose-6-phosphate dehydrogenase. Jiang P, Du W, Wang X, Mancuso A, Gao X, Wu M, Yang X. Nat Cell Biol. 2011 Mar;13(3):310-6. Epub 2011 Feb 20. 10.1038/ncb2172 PubMed 21336310
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