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Addgene

TAL-BBL1-ID21
(Plasmid #41519)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41519 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1Δ4728-2101
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 2698
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Polylinker flanked by 2 Mva1269I cut sites
  • Species
    Synthetic
  • Insert Size (bp)
    59
  • Promoter SV40

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer ASK-FN2 (CGGCCTTTTTACGGTTCCTG)
  • 3′ sequencing primer pBABE_3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TAL-BBL1-ID21 was a gift from Veit Hornung (Addgene plasmid # 41519 ; http://n2t.net/addgene:41519 ; RRID:Addgene_41519)

  • For your References section:

    A ligation-independent cloning technique for high-throughput assembly of transcription activator-like effector genes. Schmid-Burgk JL, Schmidt T, Kaiser V, Honing K, Hornung V. Nat Biotechnol. 2012 Dec 16. doi: 10.1038/nbt.2460. 10.1038/nbt.2460 PubMed 23242165
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