pcDNA3.1 JellyOp
(Plasmid #41432)

• Depositing Lab: Robert Lucas
• Publication: Bailes et al PLoS One. 2012;7(1):e30774. Epub 2012 Jan 24.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5347
• Total vector size (bp): 6373
• Modifications to backbone: An HpaI recognition site was introduced 34 bases downstream of the CMV promoter region in the pcDNA3.1 backbone to enable cloning in of 5' blunted inserts (e.g. JellyOp).
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Box jellyfish opsin
• Alt name: JellyOp
• Species: Carybdea rastonii
• Insert Size (bp): 1026
• GenBank ID: AB435549
• Promoter: CMV
• Tag / Fusion Protein:
  • 1D4 epitope at 3' end (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HpaI (destroyed during cloning)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: M13 F
• 3′ sequencing primer: M13 R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This gene was synthesised by Genscript Corp at our request and provided in a puc57 cloning vector, from which our lab constructed this pcDNA3.1 JellyOp plasmid.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The original JellyOp gene from which we designed this coding region with a 1D4 fusion was sequenced by Koyanagi et al (2008). PNAS 105:15576-15580.