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hRIP1 HA GFP wt
(Plasmid #41396)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41396 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RIP1
  • Alt name
    RIPK1
  • Alt name
    RIP
  • Species
    H. sapiens (human)
  • Mutation
    E96G
  • GenBank ID
    NM_003804
  • Entrez Gene
    RIPK1 (a.k.a. AIEFL, IMD57, RIP, RIP-1, RIP1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • GFP (C terminal on backbone)
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site n/a (unknown if destroyed)
  • 3′ cloning site n/a (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hRIP1 HA GFP wt was a gift from Francis Chan (Addgene plasmid # 41396 ; http://n2t.net/addgene:41396 ; RRID:Addgene_41396)

  • For your References section:

    Phosphorylation-driven assembly of the RIP1-RIP3 complex regulates programmed necrosis and virus-induced inflammation. Cho YS, Challa S, Moquin D, Genga R, Ray TD, Guildford M, Chan FK. Cell. 2009 Jun 12;137(6):1112-23. 10.1016/j.cell.2009.05.037 PubMed 19524513
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