hRIP1 HA GFP K45A
(Plasmid #41389)

• Depositing Lab: Francis Chan
• Publication: Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RIP1
• Alt name: RIPK1
• Alt name: RIP
• Species: H. sapiens (human)
• Mutation: Kinase Domain Mutant (K45A)
• GenBank ID: NM_003804
• Entrez Gene: RIPK1 (a.k.a. AIEFL, IMD57, RIP, RIP-1, RIP1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • GFP (C terminal on backbone)
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: n/a (unknown if destroyed)
• 3′ cloning site: n/a (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-N

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry